Research Article

Probiotics (Bifidobacterium longum) Increase Bone Mass Density and Upregulate Sparc and Bmp-2 Genes in Rats with Bone Loss Resulting from Ovariectomy

Table 3

Effect of B. longum on the mineral content of the femur.

Femur mineral content (mg/g) ShamOVXOVX + B. longum value

Ca 246.23 ± 10.14242.09 ± 5.16242.45 ± 15.260.280
Mg 33.23 ± 0.1532.44 ± 0.1232.76 ± 0.310.220
Zn 17.23 ± 2.0413.89 ± 0.6713.96 ± 1.250.241

Ten-week-old female Sprague-Dawley rats were supplemented with 1 mL of B. longum (108–109 CFU) once a day for 16 weeks, starting 2 weeks after surgery, while sham and OVX groups received 1 mL of demineralized water. At the end of the study, the calcium (Ca), magnesium (Mg), and zinc (Zn) content of the femur was analyzed. Values represent the mean ± SEM ( in each group).
No significant difference was observed between the groups based on one-way ANOVA, followed by Tukey’s post hoc test.