ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes
Table 1
Secreted proteins of unstimulated and stimulated PBLs obtained from different subjects.
Parameter
Unstimulated cells
LPS-stimulated cells
Range
(2)
= (RV(3) + LPS)/LPS
(2) = (EPA(3) + LPS)/LPS
(pg/mL)
(pg/mL)
(pg/mL)
IL-1β
0 ± 0(1)
7889 ± 1250(1)
2025–18000
2.62 ± 0.30
2.76 ± 0.94
IL-6
1 ± 1
111644 ± 19519
51300–209000
1.76 ± 0.30
2.29 ± 0.51
IL-10
1 ± 1
1279 ± 230
388–2530
1.29 ± 0.35
1.06 ± 0.21
IL-12 (p70)
0 ± 0
14 ± 7
0–73
—
—
TNF-α
0 ± 0
3499 ± 1300
418–12750
0.86 ± 0.15
1.44 ± 0.65
CCL2/MCP-1
1 ± 1
724 ± 163
363–2065
0.77 ± 0.26
0.95 ± 0.14
CCL3/MIP-1α
1 ± 1
14718 ± 2698
2720–28250
1.51 ± 0.56
1.27 ± 0.47
CCL4/MIP-1β
182 ± 31
68950 ± 12844
33300–162500
1.75 ± 0.46
0.81 ± 0.17
CCL5/RANTES
536 ± 117
6013 ± 1978
670–20300
1.55 ± 1.57
0.77 ± 0.14
CCL11/Eotaxin
28 ± 8
448 ± 82
78–878
1.16 ± 0.11
1.04 ± 0.09
CXCL8/IL-8
311 ± 35
298980 ± 50712
108800–614000
0.79 ± 0.35
2.10 ± 0.60
PGE2
121 ± 15
3437 ± 499
1680–6249
0.11 ± 0.17
2.10 ± 0.64
(1)Mean ± SEM of values obtained from PBLs of 8 different subjects (each done in duplicate). (2)Ratio: (metabolites produced by PBLs treated with substance + LPS)/metabolites produced by PBLs treated with LPS. Mean ± SD of triplicate values obtained from PBLs of 4 different subjects. (3)25 μmol/L RV, 20 μmol/L EPA.
