Use of Adipose-Derived Mesenchymal Stem Cells in Keratoconjunctivitis Sicca in a Canine Model

Figure 3

Assessment of trilineage differentiation. (a) Control. Cells maintained in control medium for 21 days. (b-c) Assessment of osteogenic differentiation. (b) Histochemical localization of ALP. Osteoinduced cells formed numerous nodules highly positive for ALP staining. (c) Positive Alizarin Red S staining by day 21 whereas red calcium nodules clearly appeared on the osteoinduced cultures. (d) Assessment of adipogenic differentiation. Positive Oil Red O staining by day 21 confirmed the presence of lipid droplets only in adipogenic-induced cells. (e-f) Assessment of chondrogenic differentiation. Histological sections of pellets after 21 days in the presence or absence of rhTGF-β1. (e) Control pellets incubated without rhTGF-β1. (f) Pellets incubated with rhTGF-β1 clearly displayed improved chondrogenesis with positive toluidine blue staining. Bars 200 μm in (a–d) and 100 μm in (e-f).

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