Use of Adipose-Derived Mesenchymal Stem Cells in Keratoconjunctivitis Sicca in a Canine Model

Figure 1

Cell morphology and proliferative curve of canine Ad-MSCs cultures. (a) In primary cultures, a large number of adherent cells with fibroblastic morphology were observed from the first day of culture, forming frequent CFUs. On secondary culture, canine Ad-MSCs appeared as spindle-shaped cells that were grown in a monolayer. Bars 200 μm. (b) Representative curve obtained with MTS cell proliferation assay at p2 showing from 24 hours an important proliferative capacity, initiating the logarithmic growth phase, and reaching its plateau phase around 14 days.

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