Effects of Partial Substitution of Fish Meal by Soybean Meal with or without Heat-Killed Lactobacillus plantarum (LP20) on Growth Performance, Digestibility, and Immune Response of Amberjack, Seriola dumerili Juveniles

Table 1

Formulation of the experimental diets (% dry matter).

Ingredient	Soybean meal (SBM) replacement, % (HK-LP Prep., %)
	SBM0	SBM15	SBM15(0.1)	SBM30	SBM30(0.1)	SBM45	SBM45(0.1)
Brown fish meal^1	61	51	51	40	40	30.5	30.5
Soybean meal^2	0	15	15	30	30	45	45
Wheat flour	10	8	8	5	5	1	1
Soybean lecithin^3	3	3	3	3	3	3	3
Pollack liver oil^4	5	5	5	5	5	5	5
Vitamin mixture^5	3	3	3	3	3	3	3
Mineral mixture^6	3	3	3	3	3	3	3
Stay-C^7	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Activated gluten^8	5	5	5	5	5	5	5
α-Cellulose	9	5.63	5.53	4.17	4.07	2.2	2.1
Amino acid premix^9	0.9	1.27	1.27	1.73	1.73	2.2	2.2
HK-LP Prep^10	0	0	0.1	0	0.1	0	0.1
Total	100	100	100	100	100	100	100

Nihon Suisan Co. Ltd (Tokyo, Japan), ²J. Oil Mills, Japan, ^3,4Riken Vitamins, Tokyo, Japan.
⁵Vitamin mixture (g kg⁻¹ diet): -carotene, 0.10; Vitamin D₃, 0.01; Menadione NaHSO₃⋅3H₂O (K₃), 0.05; DL--tochopheryl acetate (E), 0.38; thiamine-nitrate (B₁), 0.06; riboflavin (B₂), 0.19; pyridoxine-HCl (B₆), 0.05; cyanocobalamin (B₁₂), 0.0001; biotin, 0.01; inositol, 3.85; niacine (Nicotic acid), 0.77; Ca pantothenate, 0.27; folic acid, 0.01; choline chloride, 7.87; -aminobenzoic acid, 0.38; cellulose, 1.92.
⁶Mineral mixture (g kg⁻¹ diet): MgSO₄, 5.07; Na₂HPO₄, 3.23; K₂HPO₄, 8.87; Fe citrate, 1.10; Ca lactate, 12.09; Al(OH)₃, 0.01; ZnSO₄, 0.13; CuSO₄, 0.004; MnSO₄, 0.03; Ca(IO₃)₂, 0.01; CoSO₄, 0.04.
⁷L-ascrobil-2-phosphate-Mg.
⁸Glico Nutrition Company Ltd. Osaka, Japan. Commercial name: “A-glu SS”.
⁹Amino acid premix (g 100 g⁻¹ diet) at soybean meal replacement level of 15%; the mixed amino acids just as follows: lysine, 0.20; methionine, 0.17; alanine, 0.30; betaine, 0.30; glycine, 0.30. Amino acid premix (g 100 g⁻¹diet) at fish meal replacement level of 30%; the mixed amino acids just as follows: lysine, 0.46; methionine, 0.38; alanine, 0.30; betaine, 0.30; glycine, 0.30. Amino acid premix (g 100 g⁻¹diet) at fish meal replacement level of 45%; the mixed amino acids just as follows: lysine, 0.72; methionine, 0.58; alanine, 0.30; betaine, 0.30; glycine, 0.30.
¹⁰HK-LP Prep: preparation of heat-killed Lactobacillus plantarum made by House Wellness Foods Corp. (Itami, Japan).