Research Article

The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma

Figure 6

Effect of tyrosinase on intracellular reactive oxygen species (ROS) induced by raspberry ketone (RK). After HaCaT keratinocytes were treated with 50.0~100.0 units/mL tyrosinase, 1.0 mM RK, and 20.0 μM 2′,7′-dichlorofluorescin diacetate (DCFDA) for 30 min, the fluoresce intensities were measured. The black and gray columns indicate the averaged intracellular ROS levels in B16F10 treated with 0 and 1.0 mM RK, respectively. The lines on the columns show the standard deviations (). Two asterisks () indicate that the probabilities of significant levels are less than 0.01 ().