The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma

Figure 4

Effects of phenol compounds on the intracellular reactive oxygen species (ROS) level of B16F10 melanoma cells and HaCaT keratinocytes. After B16F10 and HaCaT cells were treated with 1.0 mM rhododendrol (RD), raspberry ketone (RK), hydroquinone monomethyl ether (MEHQ), p-hydroxyphenylacetamide (pHPA), 2-(p-hydroxyphenyl)isovaleric acid (2pHP-IA), or p-hydroxyphenylethanol (pHPE), and 20.0 μM 2′,7′-dichlorofluorescin diacetate (DCFDA) for 30 min, the fluoresce intensities were measured. The black and gray columns indicate the averaged intracellular ROS levels in B16F10 and HaCaT cells, respectively. The lines on the columns show the standard deviations (). One () and two () asterisks indicate that the probabilities of significance levels are less than 0.05 () and 0.01 (), respectively.