Research Article

Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media

Figure 6

(a and b) Scanning electron microscopy shows the dense fiber network of the collagen matrix with mesenchymal stem cells after 10 days of dynamic culture in the bioreactor. (c) Cross-section of collagen matrix stained with DAPI fluorescence stain shows collagen fibrous network (in green) with adipose mesenchymal stem cells (blue: nuclei of MSC) cultured in the bioreactor after 14 days of culture. (d) Fluorescence microscopy shows vital mesenchymal cells longitudinally aligned after 1-week dynamic culture in perfusion bioreactor (cell vitality stain).
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