Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
Table 2
Significant miRNA profile compared to control cases in the microarray study and validation results in real time RT-PCR. According to mean values, only those miRNAs whose “fold change” value demonstrated ± 2-fold or more expression difference were included in the study. miRNAs whose “False Discovery Rate” (FDR) corrected value <0.05 were considered significant. FDR is calculated by using CLC Main Workbench 5 (CLC Bio, Denmark). A total of 13 miRNAs showed upregulation (shown in bold) and 2 miRNAs showed downregulation.
miRNA
Microarray
High/Low Expression ()*
RT-PCR
High/Low Expression ()*
Control
ALL
Ratio
value
FDR
Control
ALL
hsa-miR-548i
1.84
22.12
12.50
3.903
0.03
23/0
0.000112
0.007942
6.15
0.590
21/0
hsa-miR-708
46.55
488.77
10.00
6.554
0.01
21/7
0.002949
19.01167
12.65
0.291
28/3
hsa-miR-181b
72.46
467.47
6.25
7.096
36/3
0.0468
48.42431
10.02
0.251
33/2
hsa-miR-449a
6.66
23.84
3.57
2.693
0.03
14/0
0.003653
0.013143
1.85
0.163
7/0
hsa-miR-146a
326.65
1135.73
3.45
8.315
1.793
35/2
0.068189
24.37251
8.48
0.008
35/1
hsa-miR-155
170.68
515.25
3.03
7.935
1.793
32/2
0.063027
31.66987
8.97
0.009
34/0
hsa-miR-181a
*
104.93
298.17
2.86
6.543
0.04
25/4
0.012341
0.766604
5.96
0.123
35/2
hsa-miR-3121
12.04
33.64
2.78
6.953
0.04
21/0
0.023803
0.001278
−4.22
0.209
0/0
hsa-miR-181a
661.35
1810.68
2.70
3.663
0.03
29/7
0.770556
80.63069
6.71
0.002
34/2
hsa-miR-128
720.72
1713.49
2.38
2.676
2.884
30/0
0.245556
11.3962
5.54
0.009
33/4
hsa-miR-1323
34.21
81.96
2.38
1.053
0.01
25/2
0.000686
0.000024
−4.84
0.199
0/0
hsa-miR-195
962.76
2251.43
2.33
9.674
0.01
28/4
0.012433
1.250504
6.65
<0.001
32/3
hsa-miR-587
33.83
69.93
2.08
5.673
0.04
17/4
0.000523
0.000146
−1.84
0.326
1/0
hsa-miR-640
91.53
45.07
−2.03
7.183
0.04
3/30
0.050227
0.000498
−6.66
0.191
0/0
hsa-miR-145
498.27
197.73
−2.52
8.095
1.793
2/35
1.201444
14.55961
3.60
0.440
26/10
FDR: false discovery rate, ALL: acute lymphoblastic leukemia.
*“High/Low Expression ()” means the number of cases in which particular miRNA expression is over the normal range. Normal range was accepted for the 95% CI of control cases.
