TY - JOUR A2 - Tomoda, Akio AU - Sun, Hongxin AU - Niisato, Naomi AU - Nishio, Kyosuke AU - Hamilton, Kirk L. AU - Marunaka, Yoshinori PY - 2014 DA - 2014/04/10 TI - Distinct Action of Flavonoids, Myricetin and Quercetin, on Epithelial Cl Secretion: Useful Tools as Regulators of Cl Secretion SP - 902735 VL - 2014 AB - Epithelial Cl secretion plays important roles in water secretion preventing bacterial/viral infection and regulation of body fluid. We previously suggested that quercetin would be a useful compound for maintaining epithelial Cl secretion at a moderate level irrespective of cAMP-induced stimulation. However, we need a compound that stimulates epithelial Cl secretion even under cAMP-stimulated conditions, since in some cases epithelial Cl secretion is not large enough even under cAMP-stimulated conditions. We demonstrated that quercetin and myricetin, flavonoids, stimulated epithelial Cl secretion under basal conditions in epithelial A6 cells. We used forskolin, which activates adenylyl cyclase increasing cytosolic cAMP concentrations, to study the effects of quercetin and myricetin on cAMP-stimulated epithelial Cl secretion. In the presence of forskolin, quercetin diminished epithelial Cl secretion to a level similar to that with quercetin alone without forskolin. Conversely, myricetin further stimulated epithelial Cl secretion even under forskolin-stimulated conditions. This suggests that the action of myricetin is via a cAMP-independent pathway. Therefore, myricetin may be a potentially useful compound to increase epithelial Cl secretion under cAMP-stimulated conditions. In conclusion, myricetin would be a useful compound for prevention from bacterial/viral infection even under conditions that the amount of water secretion driven by cAMP-stimulated epithelial Cl secretion is insufficient. SN - 2314-6133 UR - https://doi.org/10.1155/2014/902735 DO - 10.1155/2014/902735 JF - BioMed Research International PB - Hindawi Publishing Corporation KW - ER -