TY - JOUR
A2 - Otto, Thomas
AU - Roman Regueros, Sabiniano
AU - Albersen, Maarten
AU - Manodoro, Stefano
AU - Zia, Silvia
AU - Osman, Nadir I.
AU - Bullock, Anthony J.
AU - Chapple, Christopher R.
AU - Deprest, Jan
AU - MacNeil, Sheila
PY - 2014
DA - 2014/07/17
TI - Acute In Vivo Response to an Alternative Implant for Urogynecology
SP - 853610
VL - 2014
AB - Purpose. To investigate in vivo the acute host response to an alternative implant designed for the treatment of stress urinary incontinence (SUI) and pelvic organ prolapse (POP). Methods. A biodegradable scaffold was produced from poly-L-lactic acid (PLA) using the electrospinning technique. Human and rat adipose-derived stem cells (ADSCs) were isolated and characterized by fluorescence-activated cell sorting and differentiation assays. PLA scaffolds were seeded and cultured for 2 weeks with human or rat ADSCs. Scaffolds with and without human or rat ADSCs were implanted subcutaneously on the abdominal wall of rats. After 3 and 7 days, 6 animals from each group were sacrificed. Sections from each sample were analyzed by Haematoxylin and Eosin staining, Sirius red staining, and immunohistochemistry for CD68, PECAM-1, and collagen I and III. Results. Animals responded to the scaffolds with an acute macrophage response. After 7 days of implantation, there was extensive host cell penetration, new blood vessel formation, and new collagen deposition throughout the full thickness of the samples without obvious differences between cell-containing and cell-free scaffolds. Conclusions. The acute in vivo response to an alternative implant (both with and without cells) for the treatment of SUI and POP showed good acute integration into the host tissues.
SN - 2314-6133
UR - https://doi.org/10.1155/2014/853610
DO - 10.1155/2014/853610
JF - BioMed Research International
PB - Hindawi Publishing Corporation
KW -
ER -