TY - JOUR
A2 - Landa-Piedra, Abraham
AU - Talamás-Lara, Daniel
AU - Chávez-Munguía, Bibiana
AU - González-Robles, Arturo
AU - Talamás-Rohana, Patricia
AU - Salazar-Villatoro, Lizbeth
AU - Durán-Díaz, Ángel
AU - Martínez-Palomo, Adolfo
PY - 2014
DA - 2014/06/05
TI - Erythrophagocytosis in Entamoeba histolytica and Entamoeba dispar: A Comparative Study
SP - 626259
VL - 2014
AB - Entamoeba histolytica is the causative agent of human intestinal and liver amebiasis. The extraordinary phagocytic activity of E. histolytica trophozoites has been accepted as one of the virulence mechanisms responsible for their invasive capacity. The recognition of the noninvasive Entamoeba dispar as a different species has raised the question as to whether the lack of pathogenic potential of this ameba correlates with a limited phagocytic capacity. We have therefore compared the process of erythrophagocytosis in both species by means of light and video microscopy, hemoglobin measurement, and the estimation of reactive oxygen species (ROS). In the present study, we confirmed that E. dispar has lower erythrophagocytic capacity. We also observed by video microscopy a new event of erythrocyte opsonization-like in both species, being more characteristic in E. histolytica. Moreover, E. dispar showed a lower capacity to produce ROS compared with the invasive species and also showed a large population of amoebae that did not engulf any erythrocyte over time. Our results demonstrate that E. histolytica has a higher phagocytic capacity than E. dispar, including a higher rate of production of ROS in the course of ingesting red blood cells.
SN - 2314-6133
UR - https://doi.org/10.1155/2014/626259
DO - 10.1155/2014/626259
JF - BioMed Research International
PB - Hindawi Publishing Corporation
KW -
ER -