3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration

Figure 2

Live/dead staining of rACs in alginate beads. (a) P1 rACs on day 0; (b) P1 rACs in chondrogenic medium for 35 d (Ind-P1rAC); (c) P1 rACs in growth medium for 35 d (Con-P1rAC); (d) P4 rACs on day 0; (e) P4 rACs in chondrogenic medium for 35 d (Ind-P4rAC); (f) P4 rACs in growth medium for 35 d (Con-P4rAC). Fluorescent images were taken at 4x, 10x, and 20x magnifications. Green indicates live cells and red indicates dead cells.

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