3D Dynamic Culture of Rabbit Articular Chondrocytes Encapsulated in Alginate Gel Beads Using Spinner Flasks for Cartilage Tissue Regeneration

Figure 1

The setup of 3D dynamic culture. Morphology of P1 (a) and P4 (b) rACs cultured in 2D was observed under phase contrast microscope. (c) Gross view of cell-laden alginate beads; (d) SEM image of the interior microstructure of alginate beads laden with P1 rACs in chondrogenic medium for 28 d; (e) schematic illustration of suspension culture of cell-laden alginate beads in a spinner flask; (f) gross view of a spinner flask; (g) cell-laden alginate beads in a spinner flask.

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