The Modulatory Effect of Ellagic Acid and Rosmarinic Acid on Ultraviolet-B-Induced Cytokine/Chemokine Gene Expression in Skin Keratinocyte (HaCaT) Cells
Figure 4
Assessment of IL-6, IL-10, and TNF-α gene expression in HaCaT cells irradiated with UVB (100 mJ/cm2) and incubated with EA (5 μMin 0.1% DMSO) or RA (2.75 μMin 0.5% DMSO), through qRT-PCR. (a) IL-6 gene expression in cells incubated, 5 h after UVB, with EA or RA for 1 h (i.e., 1 h before peak IL-6 expression was reached after UVB exposure). mRNA was extracted 6 h after UVB exposure (Ext h6); ((b), (d)) IL-10 and TNF-α gene expression in cells incubated, 23 h after UVB, with EA or RA for 1 h (i.e., 1 h before peak cytokine expression was reached after UVB exposure). mRNA was extracted 24 h after UVB exposure (Ext h24); ((c), (e)) IL-10 and TNF-α gene expression in cells incubated, 18 h after UVB, with EA or RA for 6 h (i.e., 6 h before peak cytokine expression). mRNA was extracted 24 h after UVB exposure (Ext h24). (; ns: not statistically significant).
