Alteration of ROS Homeostasis and Decreased Lifespan in S. cerevisiae Elicited by Deletion of the Mitochondrial Translocator FLX1

Table 1

Endogenous flavin content in spheroplasts and mitochondria.

Carbon source	Strain	Spheroplasts			SCM
		FAD pmoli mg^−1	FMN pmoli·mg^−1	FAD/FMN	FAD pmoli mg^−1	FMN pmoli·mg^−1	FAD/FMN
Glycerol	WT	157 ± 7	153 ± 7	1.1	160 ± 10°	30 ± 10°	4.8
	𝑓𝑙𝑥1Δ	126 ± 11	110 ± 10	1.1	140 ± 30°	40 ± 10°	4.5
Galactose	WT	263 ± 10	189 ± 8	1.4	538 ± 32	103 ± 7	5.2
	𝑓𝑙𝑥1Δ	207 ± 8*	195 ± 8	1.1	306 ± 15*	67 ± 11*	4.8

Spheroplasts and mitochondria (SCM) were prepared from WT and 𝑓𝑙𝑥1Δ cells grown in glycerol or galactose (2%) up to the exponential growth phase (5 h). FAD and FMN content was determined in neutralized perchloric acid extracts, as described in Materials and Methods. Riboflavin amount was not relevant, and thus its value has not been reported. The means (±SD) of the flavin endogenous content determined in three experiments performed with different preparations are reported. °Data published in (Bafunno et al., 2004) [26]; statistical evaluation was carried out according to Student’s 𝑡-test (*𝑃<0.05).