TY - JOUR A2 - Ferrone, Soldano AU - Liu, Hui-Fang AU - Zhang, Hui-Jie AU - Hu, Qi-Xian AU - Liu, Xiao-Yan AU - Wang, Zhi-Quan AU - Fan, Jia-Yan AU - Zhan, Ming AU - Chen, Feng-Ling PY - 2012 DA - 2012/10/03 TI - Altered Polarization, Morphology, and Impaired Innate Immunity Germane to Resident Peritoneal Macrophages in Mice with Long-Term Type 2 Diabetes SP - 867023 VL - 2012 AB - Type 2 diabetes (T2D) is associated with perturbed innate immunity. Macrophages, bridging innate immunity and metabolic disturbances, play important roles in controlling immune homeostasis. However, the effect of long-term diabetic milieu (DM) on the functions and phenotypes of macrophages is still not clear. In this study, we used resident peritoneal macrophages (RPMs) from 5-month-old db/db mice to investigate the changes of macrophages. It was found that RPMs in db/db mice significantly reduced phagocytosis and adhesion capacity. After standardization with body weight, the number of F4/80+ RPMs markedly reduced in db/db mice, and, furthermore, the macrophages skewed to M2-polarizated macrophages. The results of morphology found that the RPMs shape of db/db mice was nearly round, but the RPMs shape of control mice was spindle-shaped and irregular. In this study, we found the cell numbers, morphology, and innate immunity functions of RPMs in 5-month-old type 2 diabetic mice (db/db mice) obtained by abdominal cavity lavage were significantly altered. Importantly, we also found the remarkably increased M2-RPMs in diabetic mice for the first time. SN - 2314-6133 UR - https://doi.org/10.1155/2012/867023 DO - 10.1155/2012/867023 JF - Journal of Biomedicine and Biotechnology PB - Hindawi Publishing Corporation KW - ER -