In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish
In vivo eGFP sensor assay for zerg-3 miRNAs. (a) zerg-3 miRNA 1 with eGFP knockdown at approximately 90%. (b) zerg-3 miRNA 2 with eGFP knockdown at approximately 45% (the results are not statically significant). (c) zerg-3 miRNA 3 with eGFP knockdown at approximately 98%. (d) zer-g3 miRNA 4 with eGFP knockdown at approximately 77%. ; . All statistical analysis was carried out using one-way ANOVA. The percentage of fluorescence emitted by the injected embryos was determined by comparison to negative control embryos (those injected with scrambled miRNA).
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