In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish

Figure 2

In vivo eGFP sensor assay for zerg-2 miRNAs. (a) zerg-2 miRNA 1 with eGFP knockdown at approximately 69%. (b) zerg-2 miRNA 2 with eGFP knockdown at approximately 73%. (c) zerg-2 miRNA 3 with eGFP knockdown at approximately 32% (the results are not statically significant). (d) zerg-2 miRNA 4 with eGFP knockdown at approximately 88%. ^∗∗𝑃<0.01. All statistical analysis was carried out using one-way ANOVA. The percentage of fluorescence emitted by the injected embryos was determined by comparison to negative control embryos (those injected with scrambled miRNA).

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