Comparison of Next-Generation Sequencing Systems

Table 3

MiSeq 150PE data.

Sample	GC	Q20	Q30
Human HPV	33.57; 33.62	98.26; 95.52	93.64; 88.52
Bacteria	61.33; 61.43	90.84; 83.86	78.46; 69.04

(1) The data in the table includes both read 1 and read 2 from paired-end sequencing.
(2) GC represents the GC content of libraries.
(3) Q20 value is the average Q20 of all bases in a read, which represents the ratio of bases with probability of containing no more than one error in 100 bases. Q30 value is the average Q30 of all bases in a read, which represents the ratio of bases with probability of containing no more than one error in 1,000 bases.