The Masticatory Contractile Load Induced Expression and Activation of Akt1/PKB in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit
Figure 2
Immunofluorescence colocalization of Akt1/PKB phosphorylated at Ser473 and at Thr308 in muscle fibers at the myotendinous areas. The nuclei in muscle fibers and tendocyte were visualized by staining of DNA with DRAQ5 (a, blue). The p-Akt1/PKB Ser473 (b, green) was localized in across sectioned muscle fibers at the MTJ. In muscle fibers at the MTJ, p-Akt1/PKB Thr308 (c, red) was identified. In same muscle fibers, p-Akt1/PKB Ser473 and Thr308 were colocalized (d, yellow). The tendon cells were negative for localizations of Ser473 as well as of Thr308. MTJ = myotendinous junction, t = tendon. Bar: (a)–(d) = 20 m.
