The Masticatory Contractile Load Induced Expression and Activation of Akt1/PKB in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit
Figure 1
The constitutive localization of the t-Akt1/PKB, phosphorylation of t-Akt1/PKB at Thr308 and at Ser473 in muscle fibers within muscle-tendon-bone unit. In comparison to the staining of t-Akt1/PKB (a, b), p-Akt1/PKB Thr308 (c, d) and Ser473 (e, f) in the peripheral muscle fibers, t-Akt1/PKB (b, MTJ), phosphorylation of Akt1/PKB at Thr308 (d, MTJ) and at Ser473 (f, MTJ) were detected with higher staining intensities in muscle fibers at the myotendinous junction (MTJ) area. The staining intensity of Akt1/PKB in peripheral muscle fibers (b, 602.70 117.05) was weakly compared to those in the area near to the MTJ (b, 1050.63 91.68). In comparison to the staining intensity of the p-Akt1/PKB Thr308 in peripheral muscle fibers (d, 319.76 74.57), the staining intensity of p-Akt1/PKB Thr308 was higher in muscle fibers near to the MTJ areas (d, 1138.85 98.99). The staining intensity of p-Akt1/PKB Ser473 in muscle fibers at the MTJ areas (f, 351.65 104.63) was greater than staining intensity of Ser473 in peripheral muscle areas (f, 1099.70 135.93). Data are mean SD; = 6; significant differences were considered at a value . MTJ = myotendinous junction, pM = peripheral muscle fibers, ab = alveolar bone, pdl = periodontal ligament, p = pulpa, d = dentin. Bars: 640 m for (a), (c), (e), 80 m for (b), (d), and (f).
