Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

Figure 1

Day wise morphological changes of immature BMDCs at low doses of rmGM-CSF. Bone marrow cells were cultured in RPMI 1640 medium with 10% FBS containing low doses of rmGM-CSF and were observed under the light inverted microscope (magnification ×40). Black arrows indicate cells with a DC-like appearance. (a) Bone marrow precursors and total cells on day 0 with 100 U/ml rmGM-CSF; (b) day 5 BMDCs with 40 U/ml rmGM-CSF, showing colony and multicellulat clusters formation and early stages of dendrites; (c) day 7 with 20 U/ml rmGM-CSF immature BMDCs with ridgy shape, and (d) fully dendrites formation showing higher immature and lower mature BMDCs at day 11 with 5 U/ml rmGM-CSF.

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