Bioinorganic Synthesis of Sodium Polytungstate/Polyoxometalate in Microbial Kombucha Media for Precise Detection of Doxorubicin

Mousavi, Seyyed Mojtaba; Hashemi, Seyyed Alireza; Bahrani, Sonia; Sadrmousavi-Dizaj, Asma; Arjmand, Omid; Omidifar, Navid; Lai, Chin Wei; Chiang, Wei-Hung; Gholami, Ahmad

doi:https://doi.org/10.1155/2022/2265108

Bioinorganic Chemistry and Applications

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Abstract Introduction Materials and Methods Results Conclusion Data Availability Conflicts of Interest Authors’ Contributions Acknowledgments References Copyright Related Articles

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Green Plasmonic Nanoparticles and Polymer Vesicles for Nanomedicine Application

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Research Article | Open Access

Volume 2022 | Article ID 2265108 | https://doi.org/10.1155/2022/2265108

Bioinorganic Synthesis of Sodium Polytungstate/Polyoxometalate in Microbial Kombucha Media for Precise Detection of Doxorubicin

Seyyed Mojtaba Mousavi,¹Seyyed Alireza Hashemi,²Sonia Bahrani,³Asma Sadrmousavi-Dizaj,⁴Omid Arjmand,³Navid Omidifar,⁵Chin Wei Lai,⁶Wei-Hung Chiang,¹and Ahmad Gholami⁷

Academic Editor: Valeria De Matteis

Received24 Sept 2021

Accepted22 Jun 2022

Published08 Aug 2022

Abstract

In this study, we have developed a new platform of polyoxometalate as a biocompatible and electrosensitive polymeric biosensor for the accurate detection of doxorubicin. For this purpose, we used a green synthesis approach using tartaric acid, glutamic acid, and kombucha solvent. Thanks to its bioinorganic components, the biogenic approach can chemically modify and improve the performance of the biosensor, which was experimentally confirmed. Our results showed excellent sensitivity (175.72 μA·μM⁻¹·cm⁻²), low detection limit (DL, 8.12 nM), and low quantification limit (QL, 0.056 μM) when the newly developed biosensor was used. The results also show that the biosynthesized biosensor has improved performance in detecting DOX in the biological fluid with an accuracy of more than 99% depending on the components used, which underlines the high efficiency of the biosensor produced. Considering the body’s physiological condition, the biosensor fabricated as a biocompatible component can show high efficiency. Therefore, its applicability for clinical use still needs to be studied in detail.

1. Introduction

Doxorubicin (DOX) is a chemotherapeutic agent used under the brand name Adriamycin to treat a variety of cancers in humans. It is used to treat different types of cancer, such as lung cancer, sarcoma, Hodgkin’s/non-Hodgkin’s lymphoma, breast cancer, and leukemia. DOX helps treat cancer through several mechanisms. Its main anticancer properties are the degradation of DNA by inhibiting topoisomerase II and the production of free radicals [1]. It has been shown that the use of this drug leads to common side effects such as bone marrow aplasia, alopecia, stomatitis, vomiting, gastrointestinal disorders, neurological disorders (hallucinations, dizziness, lightheadedness), and acute nausea due to toxicity in healthy tissues or resistance of tumor cells. Of all the side effects, cardiotoxicity is the most common complication limiting DOX. Controlling DOX concentrations in biological fluids (e.g., blood) during chemotherapy is critical to minimizing side effects. Identifying DNA damage and detecting DOX in biological fluids is applied by analytical methods such as photoelectrochemistry, mass spectrometry, capillary zone, capillary liquid chromatography, electrophoresis, and fluorescence. Although these analytical methods are accurate, they have disadvantages such as high cost and long testing times. As reported and confirmed in the literature, the electrochemical method has a high potential for DNA damage detection and is an attractive alternative approach for damage detection that has recently received more attention compared to other methods [2]. Polyoxometallates (POMs) are metal oxide aggregates with a great diversity in their structure and composition [3]. Their unique inherent properties make them promising candidates for medicine, magnetism, materials science, optics, biotechnology, and environmental sensing [4–6]. Many researchers have focused on studying polyoxometalates in medicinal chemistry [7, 8]. Many POMs, such as organotitanium-substituted heteropolytungstate, PM-26, PM-17, and PM-32, showed antitumor properties against various cancers in vitro or in vivo [9–11]. ATP generation prevented the formation of the 6-FMN (Mo₇O₂₄) complex in tumor cell mitochondria, resulting in antitumor activity. Prudent et al. found that P₂Mo₁₈O₆₂ inhibits the protein kinase CK2 [12]. In addition, Müller et al. reported that K₆H₂ [TiW₁₁CoO₄₀] could be used as an inhibitor [13], as POMs can interact with proteins, although their anticancer mechanisms have not been extensively studied. Bioimprovement of polymeric structures such as POM can enhance their performance and biocompatibility.

Biosensors have attracted considerable attention due to their potential applications, including clinical diagnostics, forensic investigations, and environmental monitoring [14, 15]. Advancements in DNA biosensors can be achieved by developing surface DNA probe immobilization in conjunction with hybridization techniques between complementary DNA sequences [16, 17]. Many studies have been conducted with extensive reports on the characterization of DNA probe immobilization [18]. DNA biosensors have a very high sensitivity to direct detection of DNA structures. In addition, signal amplification strategies can increase the sensitivity of electrochemical biosensors [19, 20]. To monitor DOX that has entered clinical blood samples, Chekin et al. designed and fabricated a sensitive sensor with high efficiency based on nitrogen-doped reduced graphene oxide and chitosan as a biocompatible natural polymer [21, 22]. The research conducted by Alarfaj et al. showed that a NiO/MgO nanocomposite modified with a wire sensor has a high potential for determining DOX in human plasma, which was experimentally demonstrated [23]. Gardikis et al. presented a novel and effective drug carrier formed by using the bacterium Lactobacillus helveticus as a microbial biosensor to monitor the encapsulation efficiency of DOX [24]. The probiotic-based approach can perform well clinically as it is considered a suitable biosensor as it shows good symbiosis throughout the human body [25]. Behravan et al. used an electrochemical sensor based on a nanocomposite of modified glassy carbon electrodes (GCE), gold (Au) nanoparticles, reduced graphene oxide (rGO), and polypyrrole (PPy) for the determination of DOX. Their results showed that the modified electrodes had a high sensitivity of 185 μA·mM⁻¹ and a low detection limit of 0.02 μM with a wide linear range of 0.02 μM and −25 mM [26]. Yaghoobi et al. used a hybrid molecular nanostructure of glutamine and doxorubicin to identify human ovarian cancer cells. This system showed high sensitivity in detecting cancer cells [27]. A novel electrochemical platform for quantifying DOX in cancer cell lysates and plasma samples based on silver nanoparticles and a chitosan-coated glassy carbon electrode was proposed by Ehsani et al. This probe successfully monitored DOX concentrations in human biofluids and B16F10 cell lysates with high sensitivity [28]. Deepa et al. described an electrochemical method for determining DOX using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The sensor studied successfully monitored quality control, clinical analysis, and other therapeutic drugs [29]. Mi et al. reported a new sensor made of ZnS quantum dots in the shell, Ag nanoparticles (NPs), and CuInSe2 in the core with satisfactory results for DOX determination [30, 31]. In this research, we designed and constructed a novel biosensor with high functionality to detect DOX concentration in a targeted system. The performance of the biosensor in a biological environment will be investigated in detail.

2. Materials and Methods

2.1. Materials

In this study, all the required materials for the synthesis of advanced photocatalysts, including sodium oxalate (Na₂C₂O₄), sodium nitrate (NaNO₃), tungsten trioxide (WO₃), ethanol (C₂H₅OH), potassium permanganate (KMnO₄), hydrogen peroxide (H₂O₂), iron (III) chloride hexahydrate (FeCl₃.6H₂O), iron (II) sulfate heptahydrate (FeSO₄.7H₂O), ammonia (NH₃), tartaric acid (C₄H₆O₆), and glutamic acid (C₅H₉NO₄), were supplied by Merck & Co.

2.2. Synthesis of Sodium Polytungstate

Sodium polytungstate (SPT) was synthesized through a multistage manufacturing process. In this regard, 0.9896 g sodium oxalate (Na₂C₂O₄), 0.4954 g sodium nitrate (NaNO₃), and 2.96 g tungsten trioxide (WO₃) were first added to 150 mL of ethanol and stirred for three hours at 65°C under reflux, according to the following reaction:

In the next step, the temperature of the resulting suspension was lowered to below 5°C in an ice bath. Then, 5 g of KMnO₄ was slowly added to the mixture and stirred at room temperature for 30 minutes (500 rpm).

The temperature of the suspension was then raised to 25°C, and 10 mL of H₂O₂ was added dropwise and very slowly to the mixture. Stirring was then carried out at 500 rpm for 30 minutes. The resulting suspension was then placed in a tube furnace and annealed at 450°C for six hours under the flow of Ar. Then the resulting powder was ground and washed with ethanol and again placed in the tube furnace at 600°C for six hours for further recrystallization, followed by washing with ethanol after treatment. The resulting powder was then placed in the oven at 650°C for 6 h. Finally, the resulting powder was washed with ethanol, dried at 100°C for two hours, and ground to obtain a fine white powder of SPT.

2.3. Modification of Sodium Polytungstate through Green Protocol

A green protocol modified the purified SPT powder to improve its photocatalysis activity and organic components. First, 4.75 g FeCl₃.6H₂O and 3.89 g FeSO₄.7H₂O were added to a vessel containing deionized water and kombucha solvent in the ratio of 320 mL: 32 mL and stirred (500 rpm) for one hour at 80°C under reflux to obtain a homogeneous suspension. To prepare the kombucha solvent, a complete kombucha SCOBY (the mother kombucha SCOBY produces a complete kombucha SCOBY after 22 days) was washed well with DI and placed in a clean vessel in a dark and humid room. Then 500 ml of DI was added, and the kombucha SCOBY was fed with sugar every five days until the pH of the suspension dropped to about 1.5. The resulting suspension was used as green feed for the modification of SPT. In the next step, 1 g of well-purified SPT powder, 1 g of tartaric acid (C₄H₆O₆), and 1 g of glutamic acid (C₅H₉NO₄) were added to the resulting suspension and then stirred for 30 minutes under the same conditions. Then 40 mL of ammonia (NH₃) was added dropwise to the resulting suspension and stirred for 24 hours at 80°C under reflux. Finally, the resulting powder was filtered with 0.22 μm filler paper and washed several times with DI until the treated PST was neutralized with tartaric acid and glutamic acid (SPT-T-G) at a pH of 7.

2.4. Characterization

All prepared samples were characterized using several instruments, including Fourier transform infrared (FTIR) spectroscopy (Tensor II, Bruker, Germany) in the frequency range of 4000-400 cm⁻¹, X-ray diffraction (XRD, Series S Max Finder Mira III, Tescan), and field-emission scanning electron microscopy (FESEM) with energy dispersive X-ray (EDX, Mira III, TESCAN).

2.5. Human Blood Sample Preparations

A phosphate buffer solution (PBS, 0.01 M) was obtained by mixing the corresponding K₂HPO₄ and KH₂PO₄. Doxorubicin was prepared in different concentrations. For this purpose, an exact amount of doxorubicin stock solution (0.2 M) was dissolved in a specific volume of buffer solution (pH = 7.4) and then stored at 4 °C in a dark place. Real samples of human blood plasma were provided by the Blood Transfusion Center (Iran) and frozen at −20°C. Then, 1 mL of H₂SO₄ (2 M) was added to the 2.0 mL of sample and centrifuged at 5000 rpm for 10 minutes to separate the plasma protein residues.

2.6. Apparatus

The electrochemical tests were carried out with a galvanostat/potentiostat AUTO LAB system (model PGSTAT302N, Netherlands) at room temperature. It was equipped with a standard cell and three electrodes, with a GC electrode (Metrohm) as a working electrode, a platinum wire (Metrohm) as a counter electrode, and Ag/AgCl (KCl 3M) (Metrohm) as a reference electrode. The data analysis was carried out with Nova 1.9 software.

3. Results and Discussions

3.1. Characterization

3.1.1. FTIR Analysis

Figure 1(a) shows the FTIR spectrum of PTS and PTS/T/G as a function of the bonds formed. It was observed that the peaks in the range of 420 to 910 cm⁻¹ correspond to the vibrations of the tungstate framework. The peaks that appeared in the FTIR spectrum of SPT can be assigned to the vibration of the W-OH group (522.14 cm⁻¹), the vibration of W-O-W (649.6 cm⁻¹), the vibration of W-O (886.67 cm⁻¹), the vibration of the H-O-H group (1687.30 cm⁻¹), and the hydroxyl functional groups (-OH) (3338.10 cm⁻¹) [19]. In addition, Figure 1(a) (red color) shows the FTIR spectrum of the SPT-T-G sample. As can be seen in the figure, the peaks in the range between 530 and 630 cm⁻¹ are associated with Fe-O vibrational stretching, confirming the presence of Fe in the structure of the composition, which covalently interacted with the SPT [32]. A sharp and intense peak appeared at 617.72 cm⁻¹, representing the formed Fe-O functional groups. Other peaks appeared at 798.91 and 880.10 cm⁻¹, indicating the W-O-W and W-O vibrations, respectively [19]. These peaks and their shifts demonstrate the successful synthesis of the biosensor, whose interaction and modification proceeded as expected. According to the FTIR spectrum analysis, other peaks have appeared, which are related to the sp2 alkene C-H band (disubstituted E) (928.29 cm⁻¹), the in-plane stretching vibration of C-H (1083.28 cm⁻¹), sp3 C-H bending (1411.70 cm⁻¹), FeOO- (1599.42 cm⁻¹), and the hydroxyl functional groups (-OH) (3186.64 cm⁻¹) [21]. A sharp and broad peak of the functional group -OH represents the interaction and combination of different types of hydroxyl functional groups. Surprisingly, the SPT-T-G was not magnetic, which can be attributed to the role of kombucha solvent as it could change the nature of the synthesized Fe-based nanoparticles. To improve the biocompatibility of iron nanoparticles, the iron nanoparticles (III) should be converted into non-magnetic iron nanoparticles (II). The kombucha solvent can play this role because it contains a large amount of vitamin C and citric acid (CA) in its solvent. The citric CA acts as a capping agent and provides control of the particle size to be obtained. In situ covering of the surface of particles with CA reduces magnetic interparticle interactions, favoring their colloidal stability and making them attractive for biomedical applications. The increase in CA leads to a reduction in particle size and a decrease in interparticle interactions.

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3.1.2. XRD Analysis

The results of the XRD analysis are shown in Figure 1(b) and Table 1. SPT was successfully synthesized with several main chemical compounds, including C₁₂O₈, W₄₈Na₁₃₆O₃₂₈, W₈Na₈O₂₈, and Na₈O₄, with monoclinic cubic, orthorhombic, and cubic crystal structures, respectively. As shown in Table 1, the SPT POM consisted mainly of the cubic structure, except C₁₂O₈, which could have been formed by annealing the chemical compounds of the SPT with ethanol as a solvent. Together with the FTIR results, these data justify the successful synthesis of SPT POM. Similar to the FTIR analysis, the XRD results of SPT modified by the green protocol (i.e., SPT-T-G) also showed the successful interaction of SPT with the introduced organic chemical compounds, which may improve the sensitivity and interaction between the developed biosensor and the molecules. In this case, SPT-T-G contained various chemical compounds, including S₁₆C₆, W₄₈Na₁₃₆O₃₂₈, W₈Na₈O₂₈, Fe₁₆, and N₁C_l1 with anorthic, cubic, orthorhombic, orthorhombic, and cubic crystal structures, respectively. The presence of the leading chemical compounds of SPT (i.e., W₄₈Na₁₃₆O₃₂₈ and W₈Na₈O₂₈) together with the introduction of nanoparticles of reduced iron (II) and materials of organic modifiers (i.e., S₁₆C₆ and N₁C_l1) in the structure of the synthesized SPT indicates that the SPT POM successfully interacted with and enhanced the selected materials. This resulted in a cubic SPT nanostructure with improved organic components, essential for the efficient absorption of pollutants from aqueous media. The noises may be referred to the nature of the arrangement of the layers mean SPT-T-G characterized by several noses by XRD. The noise will be reduced when the number of layers increases; thus, the noise can be explained. The amorphous has no sharp peaks, but the crystalline has sharp peaks and may be single or polycrystalline. Amorphous materials generally contain background noise, whereas crystalline materials contain peaks.

3.1.3. FESEM Analysis

Figure 2 shows the field-emission scanning electron microscopy (FESEM) images of SPT and SPT-T-G. As can be seen, SPT has a random morphology with different particle sizes. It can be seen that the modification of SPT with kombucha solvent, tartaric acid, and glutamic acid improved the morphology of SPT [33]. They also improved the structural characteristics of the final composition. SPT-T-G cubic has been characterized by FESEM techniques with energy dispersive X-ray (EDX). According to the FESEM images in Figure 2, the synthesized SPT-T-G particles have a cubic shape with an average particle size of about 51 nm, and their size distribution is wide. The observation of XRD and FESEM amorphous powder patterns may indicate the presence of amorphous, disordered crystalline material in the sample.

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As shown in Figure 3, SPT-T-G had a well-defined cubic structure and some particles with random morphology throughout the sample, which was consistent with the data from XRD analysis. These data suggest that the modification of SPT by the green protocol significantly improved the morphology of the developed samples. The chemical structure of tartaric acid, glutamic acid, and the proposed crystalline structure corresponding to SPT is shown in Figure 3. The XRD analysis agreed well with the experimental data, and it was also found that the chemical structure of the compounds derived from SPT is cubic and that they were chemically improved. Further details can be found in Table 1.

3.1.4. EDAX Analysis

The results of the EDAX analysis are shown in Figure 4 and Table 2. As can be seen in Figure 4 and Table 2, SPT consisted mainly of C, O, Na, and W, with W (%)/A (%) of 10.74/24.20, 31.94/54.01, 12.97/15.26, and 44.35/6.35, respectively, with O, Na, and W being the main constituents of SPT. The results of FTIR, XRD, and SEM analyses confirmed the successful synthesis of SPT with high purity. The summarised results showed that SPT-T-G contained more elements chemically compared to SPT due to the existence of more organic components in SPT formed during green synthesis and the conversion of iron nanoparticles (III) to iron nanoparticles (II) when kombucha was used as a solvent. In this case, SPT-T-G consisted of C, N, O, Na, S, Cl, Ca, Fe, and W, with Cl, S, and Fe having the highest intensities and weight fractions. These data are in good agreement with previously reported analyses (i.e., FTIR, XRD, and SEM) and confirm the interaction of SPT with the added organic modifiers.

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3.2. Electrochemical Characterization of the Modified GCE

Given the CV response of the GCE to the electroactive probe [Fe(CN)6]3-/4- (5 mM), the electrode was significantly modified (Figure 5(a)). The bare GCE exhibited a well-defined reversible electrochemical response, while the electrode modified with POM1 exhibited a high peak separation (ΔEp) of 310 mV at 100 mVs⁻¹. In this case, the POM1 materials showed slow electron transfer due to their structural effects. Furthermore, the oxidation and reduction potentials of the probe shifted to values that were more positive and negative, respectively. However, a decrease in the peak current of the probe was observed when the GCE surface was coated with POM2. They exhibited much slower electrode kinetics than POM1, where the electrode surface was completely blocked with increasing hydroxyl groups, inhibiting electron transfer between POM2 and Ferro/ferricyanide. This phenomenon could be due to the negative charge of the hydroxyl groups and the repulsion of the negatively charged [Fe(CN)6]3-/4-ions, which prevent the electrons from reaching the electrode surface. Considering a solution containing [Fe(CN)6]3-/4-ions with a frequency range of 105 to 10-2 Hz, a direct potential of 0.22 V, and an amplitude of ten mV at pH 7.4, impedance spectroscopy was performed and fundamentally evaluated to assess the ability of the surface to transfer electrons to different modified electrodes in this solution. The Nyquist plots from the EIS study and the electrical equivalent circuit obtained at the GCE electrode after each modification are shown in Figure 5(b). The R_ct value can be directly determined from the diameter of the semicircle of the Nyquist plots. It should be noted that the electron transfer resistance of the GCE, POM1-GCE, and POM2-GCE modified electrodes are close to 554.68 Ω, 212.65 Ω, and 1424.11 Ω, respectively. The modified electrodes exhibited a lower charge transfer resistance (Rct) than the bare electrode, according to the results achieved. These results mean that the electron transfer rate has increased due to the GCE coating and POM1 used. With POM2, on the other hand, the electron transfer capability decreased due to the negative charge of the hydroxyl groups and the repulsion of the negatively charged [Fe (CN)6]3-/4-ions.

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3.3. Electrocatalytic and Sensing Performance

The electrocatalytic ability of the modified electrode was evaluated using the square wave voltammetry method with Vin ranging from −0.9 to −0.3 and 0.05M PBS. As shown in Figure 6, no significant peak current was observed at the unmodified GC electrode in the electrolyte containing 0.1 M doxorubicin solution. A current signal appeared at the electrode modified with POM and POM2 due to the doxorubicin solution. These results indicate that the POM2 deposited on the GC electrode made the electrode electroactive to detect doxorubicin. Upon further analysis, it was found that many peaks appeared on the modified electrode in the electrolyte without doxorubicin, indicating that the functional groups formed on the POM2-based sensor may enhance the electrocatalytic properties of the unmodified GC electrode. The kinetics of the electrochemical pathways can be assessed by cycling through different sampling rates of the voltammograms, as shown in Figure 7. Figure 7(a) shows a regular increase in peak currents with increasing scan rate increases, suggesting that the process is controlled by diffusion [34].

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3.4. Calibration Curve

Optimal conditions were selected to detect different concentrations of doxorubicin. The current signal increased as the doxorubicin concentrations were gradually increased, as shown in Figure 8(a). In this context, a standard calibration curve was constructed by plotting the amount of doxorubicin against the magnitude of the signal current d (Figure 8(b)). The inset plot shows a linear calibration curve (I (μA) = 87.862 C (μM)−0.2865) between 0.04 and 0.55 μM. As shown in Figure 8, the POM2-based sensor showed high sensitivity (evaluated by calculating the slope of the standard curve/area of GC [35] of 175.72 μA·μM⁻¹). The limit of detection and limit of quantification were determined using the standard curve over 3 S/m and 10 S/m, respectively [36], and were 8.12 nM and 0.056 μM, respectively. The accuracy of the approach used was evaluated and analyzed concerning doxorubicin adsorption on the POM2-modified electrode. Five measurements at three concentration levels (0.1 μM, 0.3 μM, and 0.5 μM) were considered in this case. The relative standard deviation was in the range of 3.15%–5.12%, confirming the high repeatability of the sensor response for doxorubicin determination. Another GC electrode with the same surface area was used to test the robustness of the proposed approach. The relative standard deviation was 4.12% for the determination of 0.30 μM doxorubicin, demonstrating the robustness of the POM2/GCE response.

A comparison of the results of the proposed method with other methods for quantifying DOX is shown in Table 3. Hashemi et al. recently developed a polyrhodanine biosensor functionalized with graphene oxide and iron oxide nanoparticles [37]. They integrated the kombucha solvent supernatant into the system to enhance biocompatibility and sensitivity. Their nanobiosensor revealed superior sensitivity and accuracy, where the sensitivity, lower limit of detection, and lower limit of quantification were measured to be 167.62 μA·μM⁻¹·cm⁻², 0.008 μM and 0.056 μM, respectively. This system detected DOX in the human blood plasma with high accuracy (>99%). As can be seen, the present work demonstrates a reasonable detection limit, and compared to some of the publications [38, 39], it shows a lower detection limit (8.12 nM) and quantification limit (56 nM). This fact validates the suitability of our sensor to detect DOX with high sensitivity.

3.5. Analytical Application

The applicability of the fabricated electrochemical sensor was evaluated in real spiked human plasma samples. Known DOX concentrations were added to the samples, and DPV measurements were performed using POM2/GCE. The results are presented in Table 4. The recovery values confirm the high accuracy of the DOX determination and the absence of matrix effects.

4. Conclusion

This study presented a novel biosensor with excellent functionality for detecting doxorubicin for targeted treatment. Using a green synthesis approach, we prepared a biosensor based on sodium polytangstate/polyoxometalate. The newly developed biosensor showed excellent physicochemical properties due to the unique and extraordinary components used in the biosensor structure. Our results indicate that the fabricated biosensor has a high potential for detecting DOX in the biological fluid. According to the results, the developed biosensor will be very useful in detecting DOX and can contribute to targeted treatment in a biological environment.

Data Availability

All data used to support the findings of this study are included within the article.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Authors’ Contributions

SMM and SAH wrote the original draft. SB, ASD, OA, and NO contributed to data curation and conceptualization. LCW, WHC, and AG contributed to supervision. AG contributed to funding acquisition. WHC and AG reviewed and edited the final manuscript.

Acknowledgments

The authors acknowledge the Vice-Chancellor of Shiraz University of Medical Sciences, Shiraz, Iran, for giving lab facilities, characterization, and chemicals. This work was funded by the Shiraz University of Medical Sciences, Shiraz, Iran, under grant number 16596 (lastly, 98-01-36-19698).

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Copyright © 2022 Seyyed Mojtaba Mousavi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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