Research Article

Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus

Table 1

Concentration and purity of total RNA extracted from the monolayer of Vero cells infected by different viruses using TRIzol reagent (group means ± SD).

Concentration (ng/µL)Purity
Spectrophotometer (n = 3)OD 260/280OD 260/230

Uninfected Vero cells1421.27 ± 4.271.91 ± 0.081.86 ± 0.04
RVF 53354643.70 ± 27.241.93 ± 0.041.90 ± 0.04
PPR virus4067.57 ± 34.711.98 ± 0.131.97 ± 0.04
RVF G643458.97 ± 13.862.01 ± 0.091.83 ± 0.06
RVF 19833432.07 ± 8.321.96 ± 0.062.00 ± 0.04
RVF G662944.40 ± 55.451.99 ± 0.021.95 ± 0.07