Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus

<div>(a) Effect of loop primers (LP) on sensitivity and incubation duration. M-molecular ladder, 1‒30 minutes without LP; 2‒30 minutes with LP; 3‒45 minutes without LP; 4–45 minutes with LP; 5–60 minutes without LP; and 6–60 minutes with LP. (b) Specificity of the colorimetric-RVFV-UDG-RT-LAMP assay. M-100 bp molecular ladder, 1–4 RVFV strains G66, 1983, 5335, and G64, 5-PPR, 6-Capripox, 7-NTC.</div>

Advances in Virology

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Figure 7

Figure 7: Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus 

Figure 7 | Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus 