Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus

<div>Optimization of optimum conditions for RT-LAMP. (a) Optimization of the amplification temperatures for the RT-LAMP assay M-100 bp molecular ladder, 1-NTC, 2-65°C, 3-60°C, 4-55°C, and 5-50°C for a duration of one hour. (b) Optimization of the incubation duration for the RT-LAMP assay was 1–50 minutes, 2–55 minutes, 3–60 minutes, and 4–65 minutes, M-100 bp molecular ladder at 65°C.</div>

Advances in Virology

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Figure 6: Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus 

Figure 6 | Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus 