Detection of HIV-1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients
Table 3
Presence of HIV-1 proteins in HIV+ patient urinary EVs.
ID
ART
AIDS
Viral load
CD4 cells/ul
Nef
Gag
Pol
Protease
Rev
RT
Tat
Vif
p1
p24
p17
Poly
Vpu
Env
Vpr
Vif
copies/ml
22
No
224
X
X
X
X
X
X
X
X
X
X
27
Yes
AIDS
<50
134
X
28
Yes
AIDS
280100
22
X
X
X
X
X
30
Yes
AIDS
>10000
<20
X
X
X
X
X
X
X
X
X
X
41
Yes
29187
440
X
X
X
46
Yes
<50
689
X
X
X
X
45
Yes
400
345
X
X
X
X
X
48
Yes
4974
454
X
X
X
X
51
NA
NA
X
X
X
X
X
52
Yes
51
574
X
X
X
X
X
X
X
X
61
<50
655
X
X
62
Yes
AIDS
<50
232
X
63
No
2023
83
X
65
No
NA
NA
X
66
No
NA
NA
X
67
Yes
75
509
X
68
No
NA
NA
X
69
Yes
<50
187
X
70
Yes
<50
399
X
71
Yes
<50
456
X
74
NA
NA
X
86
Yes
<75
1642
X
X
X
X
103
Yes
AIDS
150
560
X
X
X
104
Yes
AIDS
77
313
X
X
108
Yes
AIDS
<50
653
X
X
X
110
Yes
<50
379
X
X
111
Yes
AIDS
<50
182
X
X
112
Yes
AIDS
>200
581
X
X
142
Yes
<50
487
X
X
X
X
X
X
X
X
173-1
Yes
<50
398
X
X
X
X
X
X
X
X
X
X
173-2
Yes
<50
398
X
X
X
X
X
X
174-1
Yes
48
315
X
X
X
X
X
X
174-2
Yes
48
315
X
X
X
X
196-1
Yes
AIDS
<50
113
X
X
X
X
X
X
X
X
X
X
X
X
X
196-2
Yes
AIDS
<50
113
X
X
X
X
X
X
X
X
X
X
X
X
X
An initial protein identification list was generated from matches with an Xcorr score versus charge state of 1.0 (+1), 1.5 (+2), and 1.7 (+3) and consensus scores greater than 10.0; NA = not available.
