Screening of Viral Pathogens from Pediatric Ileal Tissue Samples after Vaccination
Figure 2
Results of PCR confirmation of PCV-2, rotavirus A, parechovirus, echovirus, TTV, and small anellovirus from selected samples. (a) PCV-2 PCR. Lanes 1–21 are human ileum samples. R: RotaTeq vaccine; +: PK-15 cell control; N: NTC; L: 20 bp ladder. Both RotaTeq and positive control gave expected size at 84 bp. (b) Rotavirus A PCR. Sample 5 and RotaTeq gave expected band at 130 bp. No product was detected in NTC. (c) PCR results of TTV, Echovirus, parechovirus, and small anellovirus. B: blank. Sample 7 and a positive control from a previous study gave expected band size at 146 bp. Echovirus PCR from sample 5 detected an expected band size at 112 bp. Parechovirus PCR from sample 9 detected an expected band size at 113 bp. Small anellovirus PCR from samples 11 and 14 produced expected band size at 118 bp.
