Research Article
Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections
Table 2
Dynamic range of ICa.
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aThe dynamic range was determined on 10-fold serial dilution of IC spiked into lysis buffer containing DENV negative human serum as background with 4 replicates for each dilution. bNumber of copies cDNA/PCR has been calculated as 1/12.5 of the extracted RNA, assuming 100% efficiency in extraction, reverse transcription (RT), and PCR. SD: standard deviation. |