Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification

Table 3

Estimation of the accuracy of the HIV-1 RNA-based Amplix real-time PCR assay at nominal standards (20, 100, 1,000, 5,000, and 50,000 copies/mL), prepared from the AcroMetrix HIV-1 panel (applied Biosystems).

	Copies/mL	Log copies/mL ()	Mean of 5 replicates ()	Bias (%	Mean bias (%)
Standard # 1	20	1.3	1.4	4.6	3.4
Standard # 2	100	2.0	2.0	4.0
Standard # 3	1,000	3.0	3.1	2.6
Standard # 4	5,000	3.7	3.8	2.7
Standard # 5	50,000	4.7	4.8	2.9

: expected target value from the AcroMetrix HIV-1 panel.
: mean of 5 replicates obtained by the HIV-1 RNA-based Amplix real-time PCR assay.
(%) = .