The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media

Figure 2

Growth of Sulfolobus acidocaldarius and Saccharolobus solfataricus on pyroglutamate in comparison to glutamate. (a) Growth of S. acidocaldarius MW001 (dots) on 24 mM L-pyroglutamate (black). A fitted curve of cells grown on glutamate was added for better comparison (grey dashed line, see Figure 1(a) for full graph). Pyroglutamate uptake (red). (b) Growth of S. solfataricus P2 (triangles) on 24 mM L-glutamate and 14 mM L-pyroglutamate (dashed black line) and on 24 mM L-glutamate (solid black line) spiked with 14 mM L-pyroglutamate at 48 h (red triangle and arrow). A fitted curve of cells grown on glutamate was added for better comparison (grey dashed line, see Figure 1(a) for full graph). Values represent the average of four independent cultivations. Error bars represent the standard error between the four experiments. Curves were fitted with logistic regression in R.

(a)

(b)