Archaea

Research Article

Towards a Systems Approach in the Genetic Analysis of Archaea: Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii

Gene-deletion summary.


Gene	COG	Strain growth rate Hv-YPC^a	Strain growth rate CDM^a,b	Phenotype/essentiality verified

H26 (wild-type)	—	0.0227	0.0133	—
HVO_0156	1041	0.0223	0.0105
HVO_0658	0585	0.0281	0.0175
HVO_2001	0343	0.0233	0.0123	Cold-sensitivity
HVO_0236	1867	0.0238	0.0104
HVO_2906	0565
HVO_2888	1243	0.0102	0.0115	Severe growth defect
HVO_0580	0037	0.0271	0.0124
HVO_2736	1444	0.0210	0.0115	(20)
HVO_1852	0101	0.0151	0.0151	Growth defect
HVO_1594	0144	0.0107	0.0107	Growth defect
HVO_0916	1798	0.0055	0.0088	Cold-sensitivity, severe growth defect
HVO_1631	4112	0.0196	0.0097	Cold-sensitivity, slight growth defect
HVO_2477	2890	0.0219	0.0117	Cold-resistance
HVO_1716	0603	0.022	0.0143
HVO_1717	0602	0.020	0.0118
HVO_B0354	1357	0.0266	0.0128
HVO_0390	2016	0.0066	0.0064	Severe growth defect
HVO_2478	0042	0.0243	0.0075	Defined media growth defect
HVO_1475	2263	0.0266	0.0177
HVO_1105	1374	0.0216	0.0124
HVO_2008	1549	0.0245	0.0154
HVO_1105	1374,	0.025	0.0127
HVO_0574	2226	Not deleted		Predicted essential
HVO_0339	1571	Not deleted		Deleted only when gene present in trans
HVO_0697	2047	Not deleted		Predicted essential
HVO_0253	0009	Not deleted		Deleted only when gene present in trans
HVO_1173	1303	Not deleted		Predicted essential
HVO_1383	2519	Not deleted		Predicted essential
HVO_2747	1491	Not deleted		Deleted only when gene present in trans
HVO_0929		Not deleted		Predicted essential

^agrowth rates are presented hr⁻¹.
^b0.4% (v/v) glycerol used as a carbon source.
^cNot determined.