Research Article
Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection
Table 1
Calculated analytical sensitivity, specificity, accuracy, and LOD of the microspheres in miRNA detection.
| Detection status | MMS-1 | MMS-2 | MMS-3 | MMS-4 | + | − | + | − | + | − | + | − |
| Positive (TP, FP) | 20 | 1 | 20 | 3 | 20 | 3 | 20 | 3 | Negative (FN, TN) | 0 | 9 | 0 | 8 | 0 | 7 | 0 | 7 | Total | 20 | 10 | 20 | 10 | 20 | 10 | 20 | 10 | Sensitivity (%) | 100 | 100 | 100 | 100 | Specificity (%) | 90 | 80 | 70 | 70 | Accuracy (%) | 93 | 90 | 86 | 90 | LOD (pM) | 90 | 40 | 50 | 2 × 106 |
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