Advances in Polymer Technology

Review Article

Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins

Table 1

Commercial anti-PEG antibodies and recommended applications.
AntibodyIsotypeClassSourceCompanySpecificitySuggested applications
B-47GMonoclonalRabbitRabMAb®Metoxi-PEG of conjugatesELISA, WB, IHC
PEG-2-128MMonoclonalRabbitRabMAb®PEG backboneELISA, WB
RM105GMonoclonalRabbitRabMAb®Metoxi-PEGICC, WB, ELISA, IHC, flow Cyt, IP
5D6-3G1MonoclonalMouseRabMAb®PEG backboneELISA, WB
PA5-32247GPolyclonalRabbitThermo Fisher Scientific®PEGWB
09F02G3MonoclonalMouseLifespan BioSciences®PEGELISA
THE™ PEG antibodyMMonoclonalMouseLifespan BioSciences®PEG backbone and conjugatesELISA, IHC, IP, WB
E11G1MonoclonalMouseLifespan BioSciences®PEG backboneELISA
3.3G1MonoclonalMouseLifespan BioSciences®PEG backbone and conjugatesELISA, WB, flow Cyt
6.3G1MonoclonalMouseLifespan BioSciences®PEG backboneELISA, WB, flow Cyt
15-2bG2MonoclonalMouseLifespan BioSciences®Metoxi-PEGELISA, WB, flow Cyt
r33GG3MonoclonalRatLifespan BioSciences®PEG backboneELISA
AGP3MMonoclonalMouseLifespan BioSciences®PEG backboneELISA, WB, flow Cyt, IHC
rAGP6MMonoclonalMouseLifespan BioSciences®PEG backboneELISA, WB
PEGPAB-01GPolyclonalRabbitLifespan BioSciences®PEGWB
ELISA: enzyme-linked immunosorbent assay, WB: western blot, IHC: immunohistochemistry, ICC: immunocytochemistry, and IP: immunoprecipitation.