Capacity of Histoplasma capsulatum to Survive the Composting Process

Table 1

Trials of the composting reactors system leading to establish survival of H. capsulatum at the composting process after 45 days.

Contaminated reactor					Control reactor	Observations
Assay no.	Inoculum (CFU/ml)^†	Depth	Culture on mycosel	Hc100 nested PCR	NTC 5167
1	4200	Not done	Negative	Positive	Approved	Flies grew inside culture media; this situation lead to an excessive grow of bacteria in culture, which did not allow to visualize the H. capsulatum growth.
2	3800	Not done	Negative	Positive	Approved	Bacteria and other fungi had an abundant growth, which did not allow to visualize the H. capsulatum growth.
3	5800	Surface	Negative	Positive	Approved
		5 cm	Negative	Negative
		10 cm	Negative	Positive
		15 cm	Negative	Positive
4	4050	Surface	Negative	Positive	Approved	Microbiological study was carried out to the satisfaction, although the growth of H. capsulatum was not obtained.
		5 cm	Negative	Positive
		10 cm	Negative	Positive
		15 cm	Negative	Negative
5	3200	Surface	Negative	Positive	Approved
		5 cm	Negative	Negative
		10 cm	Negative	Positive
		15 cm	Negative	Positive

^†Quantification of H. capsulatum inoculum solution which was used to contaminate each reactor.