Interference of Antinuclear Antibody (ANA) in Indirect Immunofluorescence Assay (IIFA)-Based Perinuclear Antineutrophil Cytoplasmic Antibody (pANCA) Interpretation

Table 2

ANA-IIFA pattern results versus pANCA positivity.

Sr no	ANA-IIFA pattern	No of cases	Positive pANCA (%)	Cohen's kappa	-Value (at significant level <0.05)
01	Nuclear homogenous (AC1)	89	28 (31.5)		0.00001
02	Speckled (AC4/AC5)	81	9 (11.1)		0.293
03	Centromere (AC3)	45	3 (6.7)		0.101
04	Mitosis positive (spindle/centrosome) (AC24/AC25)	73	2 (2.7)		0.001
05	Dense fine speckled (DFS) 70 (AC2)	29	3 (10.3)		0.372
06	All positive	307	45 (14.7)	0.822	0.007