Interference of Antinuclear Antibody (ANA) in Indirect Immunofluorescence Assay (IIFA)-Based Perinuclear Antineutrophil Cytoplasmic Antibody (pANCA) Interpretation

Table 1

ANA blot (ENA) results (with grading) versus pANCA positivity.

S. No	ANA-blot (ENA blot) result grade	No of cases	pANCA positive (%)	Cohen's kappa	-Value (at significant level <0.05)
01	++++	3	1 (33.3)		0.4224
02	+++	23	5 (21.7)		0.4566
03	++	59	15 (25.4)		0.0248
04	+	34	8 (23.5)		0.2135
05	(+)/-	37	1 (2.7)		0.0131
	Positives (overall)	156	30 (19.2)	0.822	0.0405
	ANA-blot negative	46	3 (6.5)