The Role of Decay Accelerating Factor in Environmentally Induced and Idiopathic Systemic Autoimmune Disease
Figure 2
Anti-CD3/anti-CD28 but not phorbol 12-myristate 13-acetate (PMA)/ionomycin lowers CD55 expression of CD4+ T-cells. Lymph node cells from B10.S mice were cultured with no additives (green), Ig isotype controls (light blue), anti-CD3/anti-CD28 (5 g/mL each) (orange), or phorbol 12-myristate 13-acetate (PMA) (40 ng/mL) and ionomycin (2 g/mL) (dark blue). Cells were incubated at 37°C and 5% CO2 for 72 hr and then analyzed by flow cytometry to determine activation status (CD44hi, CD25hi) and then CD55 expression of activated cells. /group.
