Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes
Figure 4
CD4 expression and T cell size increase in T cells undergoing cell division. CFSE-loaded NOD or BALB/c spleen cells were stimulated with Con A, harvested at 24 or 72 hrs, and then counterstained with anti-CD4-PE and CD3-PE/Cy5. For FACS analysis, 100,000 events were collected. The plot represents gated CD3+ cells. (a) Temporal analysis of CD4 expression and size (FSC) among Con A blasts. (b) CD4 expression and cell division (CFSE) in Con A blasts. The mean fluorescence intensity (MFI) for CFSE () and CD4 () expression is noted for CD4+ cells at 24 and 48 hrs. (c) The pattern of CFSE expression (cell division) amongst CD4hi and CD4lo T cells from NOD or BALB/c Con A blasts 72 hrs after stimulation.
