Effect of Lidocaine and Epinephrine on Human Erythrocyte Shape and Vesiculability of Blood Cells

Table 2

Number of events within the chosen flow cytometer SSC-A/FSC-A region corresponding to platelets and EVs (Figure 1) in the isolate from platelet-rich plasma treated with L/E.

	Subject 1; (1/L)	Subject 2; (1/L)	Subject 3; (1/L)	Average; (1/L)
Control; (added PBS)	1090	1500	1330	1306
Test; (added L/E)	4820	2820	3420	3687

SSC-A/FSC-A: side scattered light/forward scattered light; EVs: extracellular vesicles; PBS: citrated and phosphate-buffered saline; L/E: lidocaine/epinephrine. Blood was donated by three volunteers with no record of disease. Plasma with added anesthetic L/E was incubated for 15 minutes prior to isolation of EVs. The region contained more than 90% of events in all density plots.