Advances in Biology

Research Article

Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Table 1

FAME compositions (%) of Chlorella ellipsoidea and Chlorococcum infusionum under P and EDTA stress.


Fatty acids	Fatty acid compositions (%)
	Chlorella ellipsoidea			Chlorococcum infusionum
	Control	0.15 g/L P	0.05 g/L EDTA	Control	0.00 g/L P	0.08 g/L EDTA

Lauric (12:0)	1.93^b ± 0.06	—	0.46^c ± 0.04	2.68^a ± 0.26	0.40^c ± 0.08	—
Myristic (14:0)	5.78^b ± 0.23	5.29^b ± 0.30	7.74^a ± 0.39	9.23^ab ± 0.32	8.63^b ± 0.40	1.97^c ± 0.30
Palmitic (16:0)	8.92^a ± 0.44	4.26^c ± 0.31	4.86^b ± 0.27	8.63^b ± 0.36	10.84^a ± 0.72	10.59^a ± 0.36
Palmitoleic (16:1)	10.12^c ± 0.19	17.21^a ± 1.13	16.24^a ± 0.79	13.99^c ± 1.19	21.49^a ± 1.06	14.53^c ± 1.11
Stearic (18:0)	2.17^c ± 0.21	9.26^a ± 0.78	2.12^c ± 0.22	3.87^d ± 0.22	10.44^c ± 0.99	17.98^a ± 0.98
Oleic (18:1)	13.73^d ± 0.77	21.62^c ± 0.94	30.05^b ± 2.16	9.52^c ± 0.31	30.32^a ± 2.68	29.80^a ± 1.43
Linoleic (18:2)	19.52^a ± 0.74	14.12^b ± 0.86	10.17^c ± 0.99	22.92^a ± 1.10	13.05^c ± 1.19	15.02^d ± 0.75
α-Linoleic (18:3)	10.84^a ± 0.73	10.88^a ± 0.36	11.08^a ± 0.13	22.02^a ± 0.75	1.81^d ± 0.29	7.88^c ± 0.50
Arachidic (20:0)	19.04^a ± 0.77	9.71^c ± 0.49	11.38^b ± 1.13	0.60^b ± 0.13	—	1.23^a ± 0.11
Arachidonic (20:4)	0.24^c ± 0.06	6.62^a ± 0.37	5.92^b ± 0.56	2.08^a ± 0.22	—	0.25^b ± 0.15
Eicosapentaenoic (20:5)	7.71^a ± 0.14	—	—	1.19^b ± 0.38	1.61^a ± 0.07	—
Behenic (22:0)	—	1.03^a ± 0.23	—	2.68^a ± 0.54	—	—
Lignoceric (24:0)	—	—	—	0.60^b ± 0.06	1.41^a ± 0.11	0.74^b ± 0.11

SFA	37.84 ± 1.71	29.55 ± 2.11	26.56 ± 2.05	28.29 ± 1.89	31.72 ± 2.19	32.51 ± 1.86
MUFA	23.85 ± 0.96	38.83 ± 2.07	46.29 ± 2.95	23.51 ± 1.50	51.81 ± 3.74	44.33 ± 2.54
PUFA	38.31 ± 1.67	31.62 ± 1.59	27.17 ± 1.68	48.21 ± 2.45	16.47 ± 1.55	23.15 ± 1.40

Values are mean ± SD, . Values in each column superscripted by different alphabets (a–d) are 559 significantly different from each other (, DMRT).