Research Article

Carbofuran Modulating Functions of Acetylcholinesterase from Rat Brain In Vitro

Table 2

Stability of rat brain AChE activity.

Days% AChE activity remainingAChE activity

01000.132000
71000.132000
1499.80.131736
2199.80.131736
2899.50.131340
3099.50.131340

Effect of storage time at −20°C on the activity of AChE from rat brain was observed by carrying out the enzyme assay employing 100 µg protein on different days as described in Section 2. The enzyme was stored in phosphate buffer (50 mM, pH 7.4) containing 0.2% Triton X-100.