Carbofuran Modulating Functions of Acetylcholinesterase from Rat Brain In Vitro

Figure 2

Effect of substrate (ATI) on the activity of AChE from rat brain was observed by assaying the enzyme at varying concentration ATI at room temperature (26 ± 2°C) as described in Section 2 employing 100 µg protein (a). The and values were calculated using the intersection of the straight line at -axis and at the negative abscissa on -axis, respectively (b). [] is rate of reaction and [] is substrate concentration.