Research Article

Amino Acid Starvation Enhances Programmed Ribosomal Frameshift in Metavirus Ty3 of Saccharomyces cerevisiae

Table 2

Gcn2p is required for the increase of PRF efficiency in response to amino acid starvation.

Yeast strains and relevant mutations% frameshift rates  ± SD
Normal growth3-AT treated

Wild type4.0 ± 0.29.2 ± 0.5
gcn2)4.0 ± 0.45.0 ± 0.5

% frameshift rates were calculated as the percent of the ratio of β-galactosidase activities expressed from the Ty3-frameshift plasmid to the β-galactosidase activities expressed from the Ty3-frame fusion plasmid in the relevant yeast transformants.
±SD: standard deviations.