Research Article

Amino Acid Starvation Enhances Programmed Ribosomal Frameshift in Metavirus Ty3 of Saccharomyces cerevisiae

Table 1

Amino acid starvation increases PRF efficiency in a partially Gcn4p dependent manner in Ty3.

Yeast strains and relevant mutations% frameshift rates  ± SD
Normal growth3-AT treated

Wild type4.0 ± 0.29.2 ± 0.5
Δgcn44.0 ± 0.37.0 ± 0.5

% frameshift rates were calculated as the percent of the ratio of β-galactosidase activities expressed from the Ty3-frameshift plasmid to the β-galactosidase activities expressed from the Ty3-frame fusion plasmid in the relevant yeast transformants.
±SD: standard deviations.